Mouse Anti-dsDNA Ig's (Total A+G+M) ELISA Kit, 96 tests, Quantitative

cat#5110

 INTENDED USE

The Mouse anti-dsDNA Total Ig ELISA Kit is an immunoassa

suitable for quantifying or titering total antibody activity (IgG, IgA

and IgM) specific for double-stranded DNA in serum or plasma.

Other biological fluids, including tissue culture medium, may be

validated for use.

 GENERAL INFORMATION

Antibodies reactive with autologous nuclear components, such as

DNA and histones, can represent an autoimmune basis for

pathological conditions such as systemic lupus erythematosis

(SLE) in humans, and in mice homozygous for the

lymphoproliferation spontaneous mutation (Faslpr), a systemic

autoimmunity with massive lymphadenopathy associated with

proliferation of aberrant T cells, arthritis and immune complex

glomerulonephritis. These conditions include elevated levels of

anti-dsDNA and other anti-nuclear antibodies (ANA) which often

increase as the animal ages. Also, the expanded use in the drug

industry of biological modifiers has been associated with

production of autoantibodies, of which mice, and possibly also

other hosts such as humans and monkeys, are susceptible. A

prototype disease in mice is lupus caused by the drug

minocycline, with elevated anti-dsDNA among other

autoantibodies and pathological conditions.

Recent investigations have focused on the role of innate immune

mechanisms, including Toll-like receptors (TLRs) and TH2

immunity, responding to the damage-associated molecular

patterns of dying cells, as underlying cause of the anti-dsDNA

type autoimmunity; these may be induced by drugs, including

vaccines and adjuvants, with aging, or with other conditions.