Human anti dsDNA IgA ELISA Kit

cat#3110

Anti-dsDNA IgA ELISA kit is an indirect ELISA for determination of IgA antibodieto dsDNA in human serum or plasma or other biological fluids. For in vitro

research use only, not for diagnostic procedures.

INTRODUCTION

The presence of serum antibodies to native ds-DNA is one of the major criterion

for systemic lupus erythematosus (SLE). These autoantibodies are rarely found in patients

with other rheumatic diseases, and their levels especially of those with complement fixing

activity, often correlate with active disease. An increase in anti-dsDNA antibodies > 30

IU/ml in less than 10 weeks in conjunction with a decrease in C4 levels is a reliable

indicator of exacerbation of SLE.

Antibody to ds-DNA are directed against the phosphate-deoxyribose backbone of

the DNA molecule, and appear to be generated due to preferential activation of specific B

cells and are not merely due to polyclonal B-cell hyperactivity prior to exacerbation of SLE.

The common DNA idiotype (Id), designated 16/6 and encode by a germ line gene, is found

in SLE and other autoimmune disease. Immunization with hu MAb 16/6 as well as with Tcell lines specific for the 16/6 Id causes SLE-like disease in mice. ADI’s ds-DNA IgG

assay, a sandwich ELISA, provides a rapid quantitative measurement of ds-DNA in serum

for the management of SLE.