3115 -Human anti ssDNA IgG ELISA Kit

The presence of serum antibodies to native ds-DNA is one of the major

criterion for systemic lupus erythematosus (SLE). These autoantibodies are

rarely found in patients with other rheumatic diseases, and their levels especially

of those with complement fixing activity, often correlate with active disease. An

increase in anti-dsDNA antibodies > 30 IU/ml in less than 10 weeks in

conjunction with a decrease in C4 levels is a reliable indicator of exacerbation of

SLE.

Antibody to ss-DNA are directed against the phosphate-deoxyribose

backbone of the DNA molecule, and appear to be generated due to preferential

activation of specific B cells and are not merely due to polyclonal B-cell

hyperactivity prior to exacerbation of SLE. The common DNA idiotype (Id),

designated 16/6 and encode by a germ line gene, is found in SLE and other

autoimmune disease. Immunization with hu MAb 16/6 as well as with T-cell lines

specific for the 16/6 Id causes SLE-like disease in mice. ADI’s ss-DNA IgG

assay, a sandwich ELISA, provides a rapid quantitative measurement of ds-

DNA in serum for the management of SLE.