Human anti-dsDNA IgG High Sensitivity ELISA Kit, 96 tests, Quantitative

Anti-ds-DNA IgG ELISA kit is based on binding of ds-DNA antibody (IgG) from serum samples to native ds-DNA immobilized on microtiter wells. After a washing step, goat anti-human IgG-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of ds-DNA IgG present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm and the concentration of anti-DNA IgG in samples is calculated on the basis of the absorbance of the negative, positive, and, calibrator standards..